Streszczenie:
In Poland seeds from grape are a waste product that is not used as a source of valuable substances for
humans. The main aim of this study was to obtain and analyze the oil from the seeds of seven grape cultivars
from the Garlicki Lamus vineyard located in Garlica Murowana (near Krakow, Poland, coordinates: 19º56’E,
50º08’N). Our work is presenting estimation of the percentage content of oil and fatty acid composition derived
from cultivars of grape seed (Leon Millot, Jutrzenka, Bianca, Regent, Seyval Blanc, Marechal Foch, Sibera). In
samples the following fatty acids have been determined: C18:2 from 76.69 % (Bianca) to 81.33 % (Marechal
Foch), C18:1 from 10.67 % (Marechal Foch) to 14.15 % (Sibera), C16:0 – from 5.05 % (Seyval Blanc) to
7.40 % (Bianca), C18:0 (stearic acid) from 2.10 % (Sibera) to 3.16 % (Seyval Blanc). On the other hand, the
lowest concentration was found for C18:3, the presence of which in measurable amounts was found in four of
the seven analyzed vine varieties (Bianca, Regent, Seyval Blanc, Sibera). Also the percentage of the oil obtained
from the seeds of the analyzed samples was measured. The maximal concentration of oil was obtained for Sibera
(12.70 ± 0.88 %) cultivar and the smallest for Marechal Foch (6.48 ± 0.49 %). Seeds obtained from grape
pomace can provide excellent raw material for the oil, which can be used in many branches of industry. Extracts
from the oil were tested for the content of total polyphenols level from 34.7mg·l-1 (Marechal Foch) to 85.1 mg·l-1
(Seyval Blanc) and total antioxidant status, which was determined by the FRAP (Ferric Reducing Antioxidant
Power) method from 19.72 μmolFe2+·l-1 (Marechal Foch) to 817.64 μmolFe2+·l-1 (Sibera). In addition,
antioxidant capacity was measured, using the method of reduction of DPPH radical reduction. The lowest
activity was measured for Regent (4.65 %) and the highest for Marechal Foch (11.62 %). There has also been
strong antioxidant potential for two of the seven studied grape seed cultivars – Seyval Blanc and Sibera.
