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Molecular Ghrelin System in the Pancreatic Acinar Cells: The Role of the Polypeptide, Caerulein and Sensory Nerves

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dc.contributor.author Gajdosz, Ryszard
dc.contributor.author Warzecha, Zygmunt
dc.contributor.author Ceranowicz, Piotr
dc.contributor.author Dembiński, Artur
dc.contributor.author Nawrot-Porąbka, Katarzyna
dc.contributor.author Link-Lenczowski, Paweł
dc.contributor.author Pierzchalski, Piotr
dc.contributor.author Jaworek, Jolanta
dc.contributor.author Kot, Michalina
dc.contributor.author Pędziwiatr, Michał
dc.contributor.author Bartuś, Krzysztof
dc.contributor.author Bonior, Joanna
dc.contributor.author Kuśnierz-Cabala, Beata
dc.contributor.author Leja-Szpak, Anna
dc.contributor.author Olszanecki, Rafał
dc.date.accessioned 2022-04-27T10:11:25Z
dc.date.available 2022-04-27T10:11:25Z
dc.date.issued 2017
dc.identifier.citation Bonior J, Ceranowicz P, Gajdosz R, Kuśnierz-Cabala B, Pierzchalski P, Warzecha Z, Dembiński A, Pędziwiatr M, Kot M, Leja-Szpak A, Nawrot-Porąbka K, Link-Lenczowski P, Olszanecki R, Bartuś K, Jaworek J. Molecular Ghrelin System in the Pancreatic Acinar Cells: The Role of the Polypeptide, Caerulein and Sensory Nerves. International Journal of Molecular Sciences. 2017; 18(5):929. https://doi.org/10.3390/ijms18050929 pl_PL
dc.identifier.issn 1422-0067
dc.identifier.uri http://hdl.handle.net/123456789/351
dc.description.abstract Ghrelin (GHRL) is an endogenous ligand for the growth hormone secretagogue receptor (GHS-R). Experimental studies showed that GHRL protects the stomach and pancreas against acute damage, but the effect of GHRL on pancreatic acinar cells was still undetermined. Aim: To investigate the effect of GHRL and caerulein on the functional ghrelin system in pancreatic acinar cells taking into account the role of sensory nerves (SN). Methods: Experiments were carried out on isolated pancreatic acinar cells and AR42J cells. Before acinar cells isolation, GHRL was administered intraperitoneally at a dose of 50 µg/kg to rats with intact SN or with capsaicin deactivation of SN (CDSN). After isolation, pancreatic acinar cells were incubated in caerulein-free or caerulein containing solution. AR42J cells were incubated under basal conditions and stimulated with caerulein, GHRL or a combination of the above. Results: Incubation of isolated acinar cells with caerulein inhibited GHS-R and GHRL expression at the level of mRNA and protein in those cells. Either in rats with intact SN or with CDSN, administration of GHRL before isolation of acinar cells increased expression of GHRL and GHS-R in those cells and reversed the caerulein-induced reduction in expression of those parameters. Similar upregulation of GHS-R and GHRL was observed after administration of GHRL in AR42J cells. Conclusions: GHRL stimulates its own expression and expression of its receptor in isolated pancreatic acinar cells and AR42J cells on the positive feedback pathway. This mechanism seems to participate in the pancreatoprotective effect of GHRL in the course of acute pancreatitis pl_PL
dc.language.iso en pl_PL
dc.publisher MDPI pl_PL
dc.rights Uznanie autorstwa 4.0 Międzynarodowe (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/deed.pl
dc.subject ghrelin pl_PL
dc.subject growth hormone secretagogues receptor type 1a pl_PL
dc.subject caerulein pl_PL
dc.subject pancreatic acinar cells pl_PL
dc.subject acute pancreatitis pl_PL
dc.subject AR42J cells pl_PL
dc.subject sensory nerves pl_PL
dc.title Molecular Ghrelin System in the Pancreatic Acinar Cells: The Role of the Polypeptide, Caerulein and Sensory Nerves pl_PL
dc.type Article pl_PL


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