Evaluation of the fat tissue oxidative stress parameters in wistar rats in fructose and high-fat diets with the addi-tion of Cornus mas L. and Aronia juice

Abstract

Observation of many irregularities in nutrition and disorders of the organ functions, which result from an excessive level of body fat motivates or even imposes the necessity of looking for new ways of pre- vention and treatment of overweight and obesity. This work presents the effect of food with the addition of fruc- tose (30%), fat (30%) and Cornus mas fruit lyophilizate (10%) and Aronia juice addition on selected parame- ters of fat tissue oxidative stress in Wistar rats. The work calculated caloric demand for the tested animals fed with the control, fructose and high-fat diet with and without the addition of Cornus mas fruit and Aronia juice. In the tested groups the total antioxidative capacity of fat tissue, determined with the FRAP method, the activ- ity of catalase, superoxide dismutase and the level of glutathione were marked. In plasma of the tested animals biochemical parameters were also marked, such as: total cholesterol, concentration of high-density lipoprotein, the level of triglycerides, concentration of urea and uric acid and glucose levels. It was observed in the con- ducted research, that an addition of Aronia juice to the high-fat diet caused a significant increase of UA con- centration in the AJ group. Cornus mas introduced to the control diet also caused a significant increase of that parameter in the CD group. The conducted experiment shows that the CAT and SOD enzyme activity in the fat tissue homogenate depended on the type of addition introduced to the base feed. The lowest use of feed and the lowest body mass increase was observed in the animal group with high-fat diet and aronia juice (AJ group).